Dynamic Microobjects Parameters Measurement Results Display with the Television Scanning Optical Microscope

V. I. Shkliarskyi, Y. M. Matiieshyn

Lviv Polytechnic National University

Television scanning optical microscope (TSOM) combined with computer support allows control of its modes in general, individual nodes, image processing and transmission capabilities, storage and playback of the data in the right form for the operator (graphs, tables, diagrams, etc.). An example of the effective use of computer technology symbiosis and measuring systems are complex CASA (Computer-aided sperm analysis). Graphic display of measurement results methods are widely used in cytophotometry to analyze the distribution of absorbing substances in the cells processes. These ways of quantitative assessment of cytological microobjects structure (MO) are based on microphotometry: histogram and topograms, calculating of the surface relief indicator and texture coefficient and so on. It is possible to represent MO in digital or gray-scale fields, as well as pseudorelief. Solving problems related to the display of the velocity vector in the form that is most visible to the operator and can give him the maximum amount of information about the behavior of MO, as well as to simplify and speed up its work, is very important. This article contains analysis ways of display the results of measurement parameters in TSOM as single dynamic MO and dynamic individual MO which are in a group of several MO moving chaotically – with variable velocity and direction of motion. The basic principles of TSOM in determining the dynamic parameters of individual MO and averaged parameters of several MO as a whole. A vector representation of measured values used in vector-cardiography, which originated in the development of electrocardiography. Presenting measure as vektorogram enables display bias in the direction of MO. The pronounced tendency of MO movement within a certain angular sector at a certain velocity rate may indicate different defects of physiological character in the structure of the MO. Examples are the different forms of violation of human sperm structure, which affect the nature of their movement velocity and the consequent loss of the ability to fertilize. MO real movement usually occurs in 3-D space. In this case, three-dimensional model is built as vektorogram. It gives researchers the complete information on the movement of MO, providing at the same time to assess the actual relationships between different components of the movement. Under the group of dynamic MO will realize several separate dynamic MO, which are within sight of TSOM have chaotic (random) motion with variable velocity and direction of movement. Often in modern medicine, microbiology, ecology and many others there is a need to analyze the dynamic parameters not only individual MO, but averaged dynamic parameters of the group as a whole. To do this successfully, you can use all the above listed ways reflect the results of measuring the dynamics of MO.
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